Molecular biology and biotechnology: a guide for students Third EditionPDF|Epub|txt|kindle电子书版本网盘下载

Molecular biology and biotechnology: a guide for students Third EditionPDF格式电子书版下载

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PARTⅠ Laying the Foundation1

1 An Overview of Biotechnology3

2 Cell Properties and Processes45

3 Genes，Genetics，and Geneticists73

4 An Overview of Molecular Biology97

5 Recombinant DNA Technology137

PARTⅡ Classroom Activities169

A.DNA Structure and Function171

6 Student Activity：Constructing a Paper DNA Model172

7 Student Activity：DNA Replication174

8 Student Activity：From Genes to Proteins176

Student Activity：Making Antisense：Regulating Gene Expression with RNA179

9 Student Activity：Sizes of the Escherichia coli and Human Genomes182

10 Student Activity：Extraction of Bacterial DNA185

B.Manipulation and Analysis of DNA187

11 Student Activity：DNA Scissors188

12 Student Activity：DNA Goes to the Races190

13 Student Activity：Restriction Analysis of Lambda DNA194

14 Student Activity：Recombinant Paper Plasmids199

15 Student Activity：Restriction Analysis Challenge202

16 Student Activity：Detection of Specific DNA Sequences：PartⅠ.Fishing for DNA205

Student Activity：Detection of Specific DNA Sequences：PartⅡ.Combining Restriction and Hybridization Analysis207

Student Activity：Detection of Specific DNA Sequences：PartⅢ.Southern Hybridization209

17 Student Activity：Paper PCR210

18 Student Activity：DNA Sequencing：The Terminators214

Reading：Chain Terminators as Antiviral Drugs218

C.Transfer of Genetic Information221

19 Student Activity：Transformation of Escherichia coli222

20 Student Activity：Conjugative Transfer of Antibiotic Resistance in Escherichia coli225

Reading：Gene Transfer and the Spread of Antibiotic Resistance228

21 Student Activity：Transduction of an Antibiotic Resistance Gene234

22 Student Activity：Agrobacterium tumefaciens：Nature’s Plant Genetic Engineer238

D.Molecular Biology and Genetics241

23 Student Activity：An Adventure in Dog Hair，Part Ⅰ242

24 Student Activity：An Adventure in Dog Hair，Part Ⅱ：Yellow Labs246

25 Student Activity：Human Molecular Genetics250

Reading：Molecular Genetics of Cancer258

26 Student Activity：Genetics in Action264

E.Genomics271

27 Student Activity：Comparing Genomes272

Exercise 1：STRs Can Cause an RFLP278

Exercise 2：PCR Can Reveal Differences at Microsatellite Loci279

Reading：Mitochondrial DNA281

28 Student Activity：Forensic DNA Typing283

Exercise 1.A Mix-Up at the Hospital285

Exercise 2.A Paternity Case285

Exercise 3.The Case of the Bloody Knife286

Reading：DNA Forensics：An Archaeological Case289

29 Student Activity：Mapping a Disease Gene290

Reading：The Human Genome Project：Its Science，Applications，and Issues299

30 Student Activity：Microarray Analysis of Genome Expression303

Reading：Personal Genomics306

F.Bioinformatics and Evolutionary Analysis of Proteins309

31 Student Activity：Testing for Amylase Activity310

32 Student Activity：Electrophoresis of Amylase Samples315

33 Student Activity：Constructing an Amylase Evolutionary Tree318

34 Student Activity：Exploring Bioinformatics322

PART Ⅲ Societal Issues329

Introduction to Societal Issues331

35 Student Activity：Facing the Forces：Science，Technology，and Society332

Reading：The Nature of Science338

36 Student Activity：Analyzing the Issues of Biotechnology：Cloning341

Reading：Are Bioengineered Foods Safe？352

37 Student Activity：The Risks of Technology：Perception，Reality，or Both？356

Reading：Biotechnology Regulation in the United States368

38 Student Activity：Making Decisions When There Is No Right Answer372

39 Student Activity：Analyzing Ethical Dilemmas in Genetic Testing377

Reading：Science，Law，and Politics381

40 Student Activity：Analyzing Ethical Dilemmas in Gene Therapy384

PART Ⅳ Appendixes389

A Worksheets390

B Laboratory Biosafety453

C Aseptic Technique455

Glossary457

Index469