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医学生物学实验 英文PDF|Epub|txt|kindle电子书版本网盘下载

医学生物学实验 英文
  • 侯燕芝主编;张海燕,刘华副主编 著
  • 出版社: 北京:高等教育出版社
  • ISBN:9787040334333
  • 出版时间:2012
  • 标注页数:108页
  • 文件大小:15MB
  • 文件页数:125页
  • 主题词:医学:生物学-实验-医学院校-教材-英文

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图书目录

Chapter 1 Introduction to the Medical Biology Laboratory1

11 Objectives of a Teaching Medical Biology Laboratory3

12 Laboratory Rules3

13 Laboratory Safety4

14 Common Laboratory Equipments5

15 Experimental Methods in Medical Biology5

16 Evaluation of Experimental Data and Laboratory Reports18

Chapter 2 Experiments in Medical Biology21

Experiment 1 The Use of Glass Pipets and Pipettors23

Experiment 2 Amphoteric Ionization and Isoelectric Point Determination of Proteins27

Experiment 3 Quantitative Determination of Protein Concentrations by Coomassie Brilliant Blue Assay29

Experiment 4 Quantitative Determination of Proteins by Folin-phenol Assay32

Experiment 5 Determination of Proteins by UV Absorption35

Experiment 6 Separation of Amino Acid Mixtures by Ion-exchange Chromatography37

Experiment 7 Separation of Serumγ-globulins by Ion-exchange Chromatography40

Experiment 8 Separation of Serum Proteins by Cellulose Acetate Membrane Electrophoresis44

Experiment 9 Separation and Purity Determination of Serumγ-globulins by SDS-polyacrylamide Gel Electrophoresis47

Experiment 10 Determination of Serum Glutamic-pyruvic Transaminase Activity52

Experiment 11 Determination of Serum Urea Nitrogen Content55

Experiment 12 Determination of Blood Glucose Concentration58

Experiment 13 Subculture of Adhesion Cells62

Experiment 14 Counting and Determination of Live/Dead Cells64

Experiment 15 Preparation ofCell Samples for Observation of Cell Morphologies67

Experiment 16 Mitosis and Meiosis of Cells70

Experiment 17 Intracellular Distribution of Biomacromolecules73

Experiment 18 Isolation of Subcellular Components77

Experiment 19 Preparation of Chromosome Samples from Mouse Bone Marrow Cells80

Experiment 20 Banding Pattern of Human Chromosomes83

Experiment 21 Colony Forming Efficiency and Total Protein Analysis of Tumor Cells86

Experiment 22 Isolation,Amplification and Identification of the β-actin Gene Fragment from Mouse Liver Cells91

Experiment 23 Purification of Target DNA and Generation of Recombinant DNA97

Experiment 24 Transformation and Screening of Recombinant DNA101

Experiment 25 Determination of Serum Proteins Using Western Blotting106

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