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医学生物学实验 英文PDF|Epub|txt|kindle电子书版本网盘下载
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- 侯燕芝主编;张海燕,刘华副主编 著
- 出版社: 北京:高等教育出版社
- ISBN:9787040334333
- 出版时间:2012
- 标注页数:108页
- 文件大小:15MB
- 文件页数:125页
- 主题词:医学:生物学-实验-医学院校-教材-英文
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图书目录
Chapter 1 Introduction to the Medical Biology Laboratory1
11 Objectives of a Teaching Medical Biology Laboratory3
12 Laboratory Rules3
13 Laboratory Safety4
14 Common Laboratory Equipments5
15 Experimental Methods in Medical Biology5
16 Evaluation of Experimental Data and Laboratory Reports18
Chapter 2 Experiments in Medical Biology21
Experiment 1 The Use of Glass Pipets and Pipettors23
Experiment 2 Amphoteric Ionization and Isoelectric Point Determination of Proteins27
Experiment 3 Quantitative Determination of Protein Concentrations by Coomassie Brilliant Blue Assay29
Experiment 4 Quantitative Determination of Proteins by Folin-phenol Assay32
Experiment 5 Determination of Proteins by UV Absorption35
Experiment 6 Separation of Amino Acid Mixtures by Ion-exchange Chromatography37
Experiment 7 Separation of Serumγ-globulins by Ion-exchange Chromatography40
Experiment 8 Separation of Serum Proteins by Cellulose Acetate Membrane Electrophoresis44
Experiment 9 Separation and Purity Determination of Serumγ-globulins by SDS-polyacrylamide Gel Electrophoresis47
Experiment 10 Determination of Serum Glutamic-pyruvic Transaminase Activity52
Experiment 11 Determination of Serum Urea Nitrogen Content55
Experiment 12 Determination of Blood Glucose Concentration58
Experiment 13 Subculture of Adhesion Cells62
Experiment 14 Counting and Determination of Live/Dead Cells64
Experiment 15 Preparation ofCell Samples for Observation of Cell Morphologies67
Experiment 16 Mitosis and Meiosis of Cells70
Experiment 17 Intracellular Distribution of Biomacromolecules73
Experiment 18 Isolation of Subcellular Components77
Experiment 19 Preparation of Chromosome Samples from Mouse Bone Marrow Cells80
Experiment 20 Banding Pattern of Human Chromosomes83
Experiment 21 Colony Forming Efficiency and Total Protein Analysis of Tumor Cells86
Experiment 22 Isolation,Amplification and Identification of the β-actin Gene Fragment from Mouse Liver Cells91
Experiment 23 Purification of Target DNA and Generation of Recombinant DNA97
Experiment 24 Transformation and Screening of Recombinant DNA101
Experiment 25 Determination of Serum Proteins Using Western Blotting106